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Direct PCR Reagents and Kits

PCR products that allow for the amplification of DNA directly from unpurified source material. Includes various kits, master mixes, enzymes, and reagents.
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31
Promotions Available

Invitrogen™ Cells-to-CT™ Bulk Fast Advanced RT Reagents
GREENER_CHOICE

Invitrogen Fast Advanced Cells-to-CT Bulk RT Reagents are separately-available, large-quantity versions of the reagents used for reverse transcription (RT) in the TaqMan Fast Advanced Cells-to-CT Kit and the SYBR Green Fast Advanced Cells-to-CT Kit.

EDGE ENCOMPASS_PREFERRED
32

Invitrogen™ TaqMan™ Cells-to-CT™ HepatoExpress™ Kit
GREENER_CHOICE

The TaqMan Cells-to-CT HepatoExpress Kit is a complete cell lysate system designed for gene expression analysis directly from hepatocytes without the need of RNA purification.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Frozen, Dry Ice
GC-Rich PCR Performance High
Form Liquid
Isolation Technology Direct Cell Lysis
For Use With (Application) Gene Expression
High-throughput Compatibility Yes
PCR Method 2-step RT-qPCR
Detection Method TaqMan
Target cDNA
Format Kit
Product Type Direct Cell Lysis to RT-qPCR Kit
Sample Type Hepatocytes, HepaRG, HepG2
Reaction Speed 10 min.
Label or Dye ROX
Purification Target RNA
Starting Material Up to 100,000 Cultured Cells
33

Invitrogen™ Cells-to-cDNA™ II Cell Lysis Buffer (10 ml Bottle)

The same lysis buffer found in the Invitrogen Cells-to-cDNA™ II Kit. Supplied in five 10 mL bottles.

ENCOMPASS_PREFERRED
34

Thermo Scientific™ Phire Animal Tissue Direct PCR Kit (without sampling tools)

Thermo Scientific Phire Animal Tissue Direct PCR Kit has been developed for amplification of DNA directly from a wide variety of animal tissues including mice, fish, birds and insects.

35

Thermo Scientific™ Template Generation System II Kit

Perform transposon-mediated insertion of primer binding sites into target DNA for sequencing or PCR amplification.

36

Applied Biosystems™ Sequencing Chemistry Installation Kit for 3500xL Series Genetic Analyzer

The 3500 platform can run a wide variety of applications

ENCOMPASS_PREFERRED
37

Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit

The CellsDirect™ One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.

ENCOMPASS_PREFERRED
For Use With (Equipment) 7500 Fast System, 7500 System, BioRad iQ5, BioRad MyiQ, Corbett RotorGene, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad iCycler iQ, Cepheid SmartCycler, MJ Chromo4, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type Cells
Reaction Speed Fast or Standard
Concentration 2X
For Use With (Application) RT-PCR
Product Line CellsDirect, Platinum, SuperScript
Detection Method Primer-probe
38

Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit with ROX, for ABI 7000, 7300, 7700, and 7900(HT) instruments

The CellsDirect One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.

ENCOMPASS_PREFERRED
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf™ MasterCycler™ RealPlex, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type Cells
Reaction Speed Fast or Standard
Concentration 2X
For Use With (Application) RT-PCR
Product Line CellsDirect, Platinum, SuperScript
Detection Method Primer-probe
39

Invitrogen™ TaqMan™ PreAmp Cells-to-CT™ Kit
GREENER_CHOICE

The TaqMan PreAmp Cells-to-CT Kit makes it possible to perform gene expression analysis directly from limited or small numbers of cultured cells without RNA purification.

ENCOMPASS_PREFERRED
40

Invitrogen™ GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit

Simple, ready-to-use, all-in-one expression vector system for CRISPR-based genome editing.

ENCOMPASS_PREFERRED
41

Invitrogen™ Cells-to-CT™ Stop Solution
GREENER_CHOICE

A component of the Cells-to-CT™ Kits.

ENCOMPASS_PREFERRED
42

Applied Biosystems™ MicroSEQ™ 500 16S rDNA PCR Kit
GREENER_CHOICE

The MicroSEQ™ 500 16S rDNA PCR Kit is the PCR component of the MicroSEQ™ 500 16S rDNA Bacterial Identification System, which provides an easy-to-use DNA sequence-based method to identify most bacteria. PCR-amplified products generated with this kit can be sequenced using the MicroSEQ™ 500 16S rDNA Sequencing Kit.

43

Invitrogen™ Fast SYBR™ Green Cells-to-CT™ Kit
GREENER_CHOICE

Ambion™ Fast SYBR™ Green Cells-to-CT™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results.

ENCOMPASS_PREFERRED
Shipping Condition Dry Ice
Content And Storage Store Stop Solution, DNase I and 20X RT Enzyme Mix at -20°C. Store Lysis Solution, 2X SYBR™ RT Buffer, Fast SYBR™ Green PCR Master Mix at 4°C. Store Nuclease-free Water at room temperature.
Purity or Quality Grade UP (Ultra Pure)
Reverse Transcriptase M-MLV
Purification Time 5 min.
Concentration 20X
For Use With (Application) RT-PCR
Detection Method SYBR
Polymerase AmpliTaq Fast DNA Polymerase
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Sample Type Cells
Reaction Speed Fast
Label or Dye SYBR Green
Hot Start Built-In Hot Start
Product Line Cells-to-CT, SYBR
44

Applied Biosystems™ MicroSEQ™ D2 rDNA Fungal PCR Kit

This kit is the PCR component of the MicroSEQ™ Fungal Identification System, which provides an easy-to-use DNA sequence-based method to identify most fungi.

45

Invitrogen™ Precision gRNA Synthesis Kit

Simple, ready-to-use, all-in-one expression vector system for CRISPR-Cas9. Transfection-ready guide RNA (gRNA) in as little as four hours.

ENCOMPASS_PREFERRED